Laboratory for extraction of natural products and synthesis of bioactive compounds

The laboratory is a part of the BioPharm Lab Complex in Sofia Tech park. The primary mission of the laboratory is to support the utilization of the existing biodiversity in Bulgaria – medicinal and aromatic plants, which are of high quality and contain active ingredients, thus stimulating sustainable development and economic growth.

  • Application of green extraction technologies for utilization of Medicinal Aromatic Plants (MAPs).
  • Fractioning and purification of extracts, investigation of the content of active ingredients and their biological activity.
  • Determination of activity profiles and development of standard procedures and documentation for phytochemical production, oriented to small and medium sized enterprises (SMEs).
  • Development of standardization and control procedures for MAPs-based products, with the purpose to guarantee the consumer’s interests.
  • Isolation of individual biologically active compounds, structure elucidation and synthesis of natural compounds analogues, possessing structural diversity and bioactivity (identification of promising compounds for development of drug candidates).

Current projects:

  • Development of a method for quantitative determination of pyrrolizidine alkaloids in dry plant material (herbs)
  • Characterization and standardization of the various types of essential oil of Melissa officinalis (Lemon Balm oil);
  • Development of new approaches for verifying the authenticity of Rose and Lavender oils;
  • Development of methods for monitoring of natural products by high performance thin layer chromatography (HPTLC);
  • Development of a method for the determination of allergens in essential oils and cosmetic formulations.

Completed projects:

Preparation of bioactive extracts from medicinal plants for the creation of innovative products

  • Development of procedures for extraction of biologically active components from medicinal plants. Preparation of pilot quantities of standardized bioactive extracts accompanied by analytical and others protocols
  • Development of methods for analysis of mixtures of natural compounds, components for incorporation in cosmetic products, and final pharmaceutical formulations.
  • Development of methodologies for analysis of natural products by high-performance thin-layer chromatography (HPTLC).
  • Development of methods and procedures for synthesis, as well as obtaining of specified quantities of organic compounds ordered by pharmaceutical companies.
  • Development of methods for the control of compounds in plant extracts (eg caffeic acid, rosmarinic acid, quercetin, etc.);
  • Authentication of rose oil by GC-MS analysis.
  • GC with QTOF MS / MS detector
  • GC with FID and MS detectors
  • Triple Quadrupole Mass Spectrometer – LCMS-8045
  • Liquid Chromatograph with Diode Array Detector and Single Quadrupole Mass Spectrometer – LCMS-2020
  • Liquid Chromatograph with Diode Array Detector and Fluorescence Detector – Nexera X2.
  • Apparatuses for automatic application, development, functionalization and densitometry of TLC / HPTLC plates;
  • Flash chromatographs;
  • Multi-purpose 5 liter reactor;
  • Laboratory equipment for extraction of natural products and synthesis of organic compounds (reactors, rotary vacuum evaporators, vacuum installations, etc.)
Prof. Vladimir Dimitrov

Dissertation (Dr. rer. Nat.) at the Technical University of Leuna-Merseburg; 2002 “Doctor of chemical sciences” HAC; 1989-1990 Scholarship Foundation Alexander von Humboldt; 1992 and 1998-2001 researcher at the University of Zurich;

Numerous lectures at foreign universities and international conferences; Numerous articles in international journals;

Many completed contracts in the EU framework programs, international funds, etc; contracts with pharmaceutical companies. Contracts in the EU framework programs, international funds, etc .; contracts with pharmaceutical companies.

Sofia, bul. Tsarigradsko Shousse 111
Laboratory Complex, Floor 2



Phone: + 359 889 900 614

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