Laboratory

In Vitro – laboratory for evaluation of biological activity and toxicity

About the Laboratory

The laboratory provides state of the art equipment in the following areas: cell cultures, DNA analyses, microbiology, pathogenic microorganisms, GMOs, ecotoxicological research, in vitro plant cultures etc.

The laboratory is part of the Biopharmaceutical Laboratory Complex and its main task is evaluation of the biological activity and toxicity.

Activities

Current Activities

Human and mammalian cell cultures for testing and characterization of biological activities of various extracts and purified bioactive compounds.
Antimicrobial activities of various extracts and individual biologically active compounds.
Plant cell and tissue cultures for propagation and maintaining of valuable MAPs accessions and cell lines.
Bacterial, yeast and fungi cultures for obtaining and biotransformation of bioactive compounds and production of recombinant proteins.
Various applications of molecular biology and recombinant DNA methods including molecular marker applications for genotyping and genetic authenticity analysis, gene expression analysis, tailor-made expression vectors construction and various expression studies.
Ecotoxicology testing and valuation of various industry products, wastes, bioactive extracts and compounds.

Services and Equipment

Services

Storage and maintenance of the microorganism collection
Isolation of mononuclear cells
In vitro testing of cytotoxicity and antioxidant activity of sample biologically active compounds on human and animal cell lines.
Development of individual experimental projects
Microbiological testing of cosmetic and food products.

Equipment

Multi-detector hybrid system for toxicity and mechanism of action assessment of biologically active substances on cellular model systems Cell Imaging Multi-Mode Reader – Cytation 3
Multifors 2 parallel cultivation bioreactor
Biological Safety Cabinet Class 2
Multi-gas Incubator (CO2, O2 & N2)
Ultra-low temperature (ULT) freezer 150 ° C
Automatic anaerobic chamber
Freeze Dryers
Analytical balance
Fluorescence Microplate Reader
UV/vis spectrometer
Fast protein liquid chromatography
Flow cytometer
Gradient PCR Machine
QPCR Machine
Trinocular Epi-Flourescence /Brightfield Compound Microscope
Cooling Centrifuge

Scientific Publications

Related Scientific Publications

No related scientific publications found.

Management

Prof. Ivan Atanasov

Scientific Areas:

Molecular Genetics; Cloning, Characterization and Expression of Genes; Recombinant Proteins; Molecular Markers; Plant Genetic Resources; Plant Biotechnology

History:

In 2012, he defended his doctoral degree and earned a Doctorate in Biological Sciences from the Agrobioinstitute Sofia, with a dissertation on the topic 'Cloning, characterization, and expression of genes in plants: New experimental methods, applications, and perspectives'. Since 2013, he has been a professor and head of the Molecular Genetics group at the Agrobioinstitute.

Specializations: Technical University Braunschweig, Germany, 2014; University of Manchester, United Kingdom, 2005-2008; International Centre for Genetic Engineering and Biotechnology (ICGEB, New Delhi), India, 2001; Ecole Normale Superieure de Lyon, France, 1998-1999; Life Science Center, Japan Tobacco Inc., 1994-1996.

More than 80 articles and over 15 completed projects in the field of molecular genetics and plant biotechnology.

Phone: +359 889 900 614
+359 889 900 603

Email:
office@sofiatech.bg

The project “Science and Technology Park” Sofia is funded by
OP “Innovations and Competitiveness”

Sofia Tech Park is part of:

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