Extraction of Natural Products and Synthesis of Bioactive Compounds

About the Laboratory

The primary mission of the laboratory is to support the utilization of the existing biodiversity in Bulgaria – medicinal and aromatic plants, which are of high quality and contain active ingredients, thus stimulating sustainable development and economic growth.

The laboratory is a part of the BioPharm Lab Complex in Sofia Tech park.


Current Activities
  • Application of green extraction technologies for utilization of Medicinal Aromatic Plants (MAPs).
  • Fractioning and purification of extracts, investigation of the content of active ingredients and their biological activity.
  • Determination of activity profiles and development of standard procedures and documentation for phytochemical production, oriented to small and medium sized enterprises (SMEs).
  • Development of standardization and control procedures for MAPs-based products, with the purpose to guarantee the consumer’s interests.
  • Isolation of individual biologically active compounds, structure elucidation and synthesis of natural compounds analogues, possessing structural diversity and bioactivity (identification of promising compounds for development of drug candidates).

Services and Equipment

  • Development of procedures for extraction of biologically active components from medicinal plants. Preparation of pilot quantities of standardized bioactive extracts accompanied by analytical and others protocols.
  • Development of methods for analysis of mixtures of natural compounds, components for incorporation in cosmetic products, and final pharmaceutical formulations.
  • Development of methodologies for analysis of natural products by high-performance thin-layer chromatography (HPTLC).
  • Development of methods and procedures for synthesis, as well as obtaining of specified quantities of organic compounds ordered by pharmaceutical companies.
  • Development of methods for the control of compounds in plant extracts (eg caffeic acid, rosmarinic acid, quercetin, etc.)
  • Authentication of rose oil by GC-MS analysis.
  • GC with QTOF MS / MS detector;
  • GC with FID and MS detectors;
  • Triple Quadrupole Mass Spectrometer – LCMS-8045;
  • Liquid Chromatograph with Diode Array Detector and Single Quadrupole Mass Spectrometer – LCMS-2020;
  • Liquid Chromatograph with Diode Array Detector and Fluorescence Detector – Nexera X2;
  • Apparatuses for automatic application, development, functionalization and densitometry of TLC / HPTLC plates;
  • Flash chromatographs;
  • Multi-purpose 5 liter reactor;
  • Laboratory equipment for extraction of natural products and synthesis of organic compounds (reactors, rotary vacuum evaporators, vacuum installations, etc.);

Scientific Publications

Related Scientific Publications
No related scientific publications found.


Prof. Vladimir Dimitrov

Bulgarian Academy of Sciences

Scientific Areas:

Development of stereoselective methods for the synthesis of bioactive compounds, natural compounds, and analogues, as well as extractions of natural products using supercritical carbon dioxide


In 1981, he defended his doctoral degree (Dr. rer. nat, magna cum laude) at the Technical University of Leuna-Merseburg, Germany. From 1989 to 1990, he was a scholar of the Alexander von Humboldt Foundation, and from 1992 to 2001 he was a researcher at the University of Zurich.

Delivered numerous lectures at foreign universities and international conferences, along with contributing numerous articles to international journals.

Completed numerous contracts within EU framework programs, international funds, etc., as well as with pharmaceutical companies.