In Vitro – laboratory for evaluation of biological activity and toxicity

About the Laboratory

The laboratory provides state of the art equipment in the following areas: cell cultures, DNA analyses, microbiology, pathogenic microorganisms, GMOs, ecotoxicological research, in vitro plant cultures etc.

The laboratory is part of the Biopharmaceutical Laboratory Complex and its main task is evaluation of the biological activity and toxicity.


Current Activities
  • Human and mammalian cell cultures for testing and characterization of biological activities of various extracts and purified bioactive compounds.
  • Antimicrobial activities of various extracts and individual biologically active compounds.
  • Plant cell and tissue cultures for propagation and maintaining of valuable MAPs accessions and cell lines.
  • Bacterial, yeast and fungi cultures for obtaining and biotransformation of bioactive compounds and production of recombinant proteins.
  • Various applications of molecular biology and recombinant DNA methods including molecular marker applications for genotyping and genetic authenticity analysis, gene expression analysis, tailor-made expression vectors construction and various expression studies.
  • Ecotoxicology testing and valuation of various industry products, wastes, bioactive extracts and compounds.

Services and Equipment

  • Storage and maintenance of the microorganism collection
  • Isolation of mononuclear cells
  • In vitro testing of cytotoxicity and antioxidant activity of sample biologically active compounds on human and animal cell lines.
  • Development of individual experimental projects
  • Microbiological testing of cosmetic and food products.
  • Multi-detector hybrid system for toxicity and mechanism of action assessment of biologically active substances on cellular model systems Cell Imaging Multi-Mode Reader – Cytation 3
  • Multifors 2 parallel cultivation bioreactor
  • Biological Safety Cabinet Class 2
  • Multi-gas Incubator (CO2, O2 & N2)
  • Ultra-low temperature (ULT) freezer 150 ° C
  • Automatic anaerobic chamber
  • Freeze Dryers
  • Analytical balance
  • Fluorescence Microplate Reader
  • UV/vis spectrometer
  • Fast protein liquid chromatography
  • Flow cytometer
  • Gradient PCR Machine
  • QPCR Machine
  • Trinocular Epi-Flourescence /Brightfield Compound Microscope
  • Cooling Centrifuge

Scientific Publications

Related Scientific Publications
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Prof. Ivan Atanasov

Scientific Areas:

Molecular Genetics; Cloning, Characterization and Expression of Genes; Recombinant Proteins; Molecular Markers; Plant Genetic Resources; Plant Biotechnology


In 2012, he defended his doctoral degree and earned a Doctorate in Biological Sciences from the Agrobioinstitute Sofia, with a dissertation on the topic 'Cloning, characterization, and expression of genes in plants: New experimental methods, applications, and perspectives'. Since 2013, he has been a professor and head of the Molecular Genetics group at the Agrobioinstitute.

Specializations: Technical University Braunschweig, Germany, 2014; University of Manchester, United Kingdom, 2005-2008; International Centre for Genetic Engineering and Biotechnology (ICGEB, New Delhi), India, 2001; Ecole Normale Superieure de Lyon, France, 1998-1999; Life Science Center, Japan Tobacco Inc., 1994-1996.

More than 80 articles and over 15 completed projects in the field of molecular genetics and plant biotechnology.